CLIC1

CLIC1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1K0M, 1K0N, 1K0O, 1RK4, 3O3T, 3P8W, 3P90, 3QR6, 3SWL, 3TGZ, 3UVH, 4IQA, 4JZQ, 4K0G, 4K0N
Identifiers
Aliases	CLIC1, G6, NCC27, chloride intracellular channel 1, CL1C1, CLCNL1
External IDs	OMIM: 602872; MGI: 2148924; HomoloGene: 20343; GeneCards: CLIC1; OMA:CLIC1 - orthologs
Gene location (Human) Chr. Chromosome 6 (human) Band 6p21.33 Start 31,730,581 bp End 31,739,763 bp
Gene location (Mouse) Chr. Chromosome 17 (mouse) Band 17 B1|17 18.58 cM Start 35,268,942 bp End 35,277,725 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in granulocyte blood monocyte mucosa of transverse colon rectum gallbladder islet of Langerhans olfactory zone of nasal mucosa placenta right lung Top expressed in corneal stroma granulocyte superior surface of tongue thymus endothelial cell of lymphatic vessel pyloric antrum lip mucous cell of stomach dermis cervix More reference expression data BioGPS More reference expression data
Gene ontology Molecular function voltage-gated ion channel activity protein binding chloride channel activity cadherin binding glutathione transferase activity Cellular component cytoplasm integral component of membrane vesicle nuclear membrane blood microparticle nuclear envelope membrane plasma membrane chloride channel complex mitochondrion brush border perinuclear region of cytoplasm extracellular exosome nucleus extracellular space Biological process regulation of ion transmembrane transport ion transport regulation of cell cycle regulation of mitochondrial membrane potential positive regulation of osteoblast differentiation chloride transport platelet aggregation signal transduction chloride transmembrane transport glutathione metabolic process transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1192 114584 Ensembl ENSG00000213719 ENSG00000223639 ENSG00000226248 ENSG00000226651 ENSG00000226417 ENSG00000230685 ENSG00000206394 ENSMUSG00000007041 UniProt O00299 Q9Z1Q5 RefSeq (mRNA) NM_001288 NM_001287593 NM_001287594 NM_033444 RefSeq (protein) NP_001274522 NP_001274523 NP_001279 NP_254279 Location (UCSC) Chr 6: 31.73 – 31.74 Mb Chr 17: 35.27 – 35.28 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Chloride intracellular channel protein 1 is a protein that in humans is encoded by the CLIC1 gene.

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.

CLIC1 is highly expressed in murine and human microglia, the innate immune cells of the central nervous system. It regulates the motility and ramification of microglial processes, which are essential for continuous surveillance of the brain parenchyma. CLIC1 is also important for the inflammatory potential of microglia by controlling the NLRP3-dependent release of interleukin-1β. Contrary to its name, these functions are not mediated by CLIC1’s ion channel activity but depend on its scaffold and enzymatic properties.

Interactions

CLIC1 has been shown to interact with TRAPPC2.

See also

• Chloride channel

Further reading

• Singh H (2010). "Two decades with dimorphic Chloride Intracellular Channels (CLICs)". FEBS Letters. 584 (10): 2112–21. Bibcode:2010FEBSL.584.2112S. doi:10.1016/j.febslet.2010.03.013. PMID 20226783. S2CID 21056278.
• Okubo K, Hori N, Matoba R, et al. (1994). "Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression". Nat. Genet. 2 (3): 173–9. doi:10.1038/ng1192-173. PMID 1345164. S2CID 35309579.
• Frigerio JM, Berthézène P, Garrido P, et al. (1995). "Analysis of 2166 clones from a human colorectal cancer cDNA library by partial sequencing". Hum. Mol. Genet. 4 (1): 37–43. doi:10.1093/hmg/4.1.37. PMID 7711732.
• Singh H, Ashley RH (2006). "Redox Regulation of CLIC1 by Cysteine Residues Associated with the Putative Channel Pore". Biophys. J. 90 (5): 1628–1638. Bibcode:2006BpJ....90.1628S. doi:10.1529/biophysj.105.072678. PMC 1367314. PMID 16339885.
• Liew CC, Hwang DM, Fung YW, et al. (1994). "A catalogue of genes in the cardiovascular system as identified by expressed sequence tags". Proc. Natl. Acad. Sci. U.S.A. 91 (22): 10645–9. Bibcode:1994PNAS...9110645L. doi:10.1073/pnas.91.22.10645. PMC 45078. PMID 7938007.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Heiss NS, Poustka A (1997). "Genomic structure of a novel chloride channel gene, CLIC2, in Xq28". Genomics. 45 (1): 224–8. doi:10.1006/geno.1997.4922. PMID 9339381.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Tulk BM, Edwards JC (1998). "NCC27, a homolog of intracellular Cl⁻ channel p64, is expressed in brush border of renal proximal tubule". Am. J. Physiol. 274 (6 Pt 2): F1140–9. doi:10.1152/ajprenal.1998.274.6.F1140. PMID 9841507. S2CID 39652235.
• Chuang JZ, Milner TA, Zhu M, Sung CH (1999). "A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons". J. Neurosci. 19 (8): 2919–28. doi:10.1523/JNEUROSCI.19-08-02919.1999. PMC 6782274. PMID 10191309.
• Ribas G, Neville M, Wixon JL, et al. (1999). "Genes encoding three new members of the leukocyte antigen 6 superfamily and a novel member of Ig superfamily, together with genes encoding the regulatory nuclear chloride ion channel protein (hRNCC) and an N omega-N omega-dimethylarginine dimethylaminohydrolase homologue, are found in a 30-kb segment of the MHC class III region". J. Immunol. 163 (1): 278–87. doi:10.4049/jimmunol.163.1.278. PMID 10384126.
• Berryman M, Bretscher A (2000). "Identification of a Novel Member of the Chloride Intracellular Channel Gene Family (CLIC5) That Associates with the Actin Cytoskeleton of Placental Microvilli". Mol. Biol. Cell. 11 (5): 1509–21. doi:10.1091/mbc.11.5.1509. PMC 14863. PMID 10793131.
• Tonini R, Ferroni A, Valenzuela SM, et al. (2000). "Functional characterization of the NCC27 nuclear protein in stable transfected CHO-K1 cells". FASEB J. 14 (9): 1171–8. doi:10.1096/fasebj.14.9.1171. PMID 10834939. S2CID 30652670.
• Valenzuela SM, Mazzanti M, Tonini R, et al. (2001). "The nuclear chloride ion channel NCC27 is involved in regulation of the cell cycle". J. Physiol. 529 (3): 541–52. doi:10.1111/j.1469-7793.2000.00541.x. PMC 2270212. PMID 11195932.
• Harrop SJ, DeMaere MZ, Fairlie WD, et al. (2002). "Crystal structure of a soluble form of the intracellular chloride ion channel CLIC1 (NCC27) at 1.4-A resolution". J. Biol. Chem. 276 (48): 44993–5000. doi:10.1074/jbc.M107804200. PMID 11551966.
• Warton K, Tonini R, Fairlie WD, et al. (2002). "Recombinant CLIC1 (NCC27) assembles in lipid bilayers via a pH-dependent two-state process to form chloride ion channels with identical characteristics to those observed in Chinese hamster ovary cells expressing CLIC1". J. Biol. Chem. 277 (29): 26003–11. doi:10.1074/jbc.M203666200. PMID 11978800.
• Shanks RA, Larocca MC, Berryman M, et al. (2002). "AKAP350 at the Golgi apparatus. II. Association of AKAP350 with a novel chloride intracellular channel (CLIC) family member". J. Biol. Chem. 277 (43): 40973–80. doi:10.1074/jbc.M112277200. PMID 12163479.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Fan L, Yu W, Zhu X (2003). "Interaction of Sedlin with chloride intracellular channel proteins". FEBS Lett. 540 (1–3): 77–80. Bibcode:2003FEBSL.540...77F. doi:10.1016/S0014-5793(03)00228-X. PMID 12681486. S2CID 8573651.
• Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6". Nature. 425 (6960): 805–11. Bibcode:2003Natur.425..805M. doi:10.1038/nature02055. PMID 14574404.
• Littler DR, Harrop SJ, Fairlie WD, et al. (2004). "The intracellular chloride ion channel protein CLIC1 undergoes a redox-controlled structural transition". J. Biol. Chem. 279 (10): 9298–305. doi:10.1074/jbc.M308444200. PMID 14613939.
• Singh H, Cousin MA, Ashley RH (2007). "Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin". FEBS J. 274 (24): 6306–6316. doi:10.1111/j.1742-4658.2007.06145.x. PMID 18028448. S2CID 22494250.

External links

• CLIC1+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
• Human CLIC1 genome location and CLIC1 gene details page in the UCSC Genome Browser.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.